Bead populations come in two sizes and differing levels of APC fluorescence, allowing them to be clearly distinguished from each other.

Please click on the software tab above to learn more about the program options and to request account access.īioLegend's LEGENDplex™ is a bead-based immunoassay that utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies. The assay can be performed in a 96-well filter plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.įor analysis of LEGENDplex™ assays, BioLegend offers free of charge data analysis software. If you would like to combine specificities across different predefined panels, i.e., Human Cytokine and Human Chemokine, please visit our custom panels tab at the top of this page.Įach kit provides sufficient reagents for at least 100 tests.

All LEGENDplex™ users are also welcome to request access to the Data Analysis Software Suite regardless of any legacy downloadsīioLegend's LEGENDplex™ kits are provided as predefined panels, ranging from 3 to 14 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system.

In the interim, the legacy programs may be downloaded, if desired, by following the instructions located in the legacy programs section of the software tab.

BioLegend will no longer be supporting the legacy programs starting in early 2021.

The dongle is the license key to the legacy software programs.

If your LEGENDplex™ kit contains a USB software dongle Please follow further instructions in the email to setup your account password

You will receive an email notifying you of account creation within 24 hours.

Click on the ‘Software’ tab located above.

To request access to the LEGENDplex™ Data Analysis Software Suite:

The CoA will display the assay’s top standard concentrations (required for data analysis).

For Mix and Match assays: enter the lot # listed on the vial of recombinant assay standards.

For Pre-defined Panels: enter the lot # listed on the assay label found on the kit packaging.

Enter the lot # for your specific LEGENDplex™ assay to open an electronic version of the document.

Click the ‘Get CoA’ button below to navigate to the CoA Lookup Tool.

To access assay certificates of analysis: Click on the ‘View Manual’ icon to open an electronic version in a new browser tab.Use the ‘Pre-defined Panels’ or ‘Mix and Match’ tabs located above to navigate to and click on your specific assay.To access user manuals for specific LEGENDplex™ panels: Please read the following information carefully.

Note: In an effort to reduce paper waste, LEGENDplex ™ assays no longer contain paper copies of user manuals or certificates of analysis.

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